An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples

Roeltje R. Maas, Klara Soukup, Florian Klemm, Mara Kornete, Robert L. Bowman, Romain Bedel, Damien N. Marie, Ángel F. Álvarez-Prado, Danny Labes, Anne Wilson, Jean-Philippe Brouland, Roy T. Daniel, Monika E. Hegi, Johanna A. Joyce

Published: 2021-08-29 DOI: 10.1038/s41596-021-00594-2

tissue processing

RNA sequencing

brain tumor

immune microenvironment

cell populations

AI 解读

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Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Human tissue samples represent an invaluable source of information for the analysis of disease-specific cellular alterations and their variation between different pathologies. In cancer research, advancing a comprehensive understanding of the unique chara
Introduction
Materials
Procedure
Troubleshooting
Timing
- Steps that need to be performed on the day of tissue reception, as soon as possible post-resection
- Steps that can be performed at a later time point
Anticipated results
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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An integrated pipeline for comprehensive analysis of immune cells in human brain tumor clinical samples

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