evo视讯(中国)

Immunofluorescence

Pietro De Camilli, William Hancock-Cerutti

Published: 2022-04-18 DOI: 10.17504/protocols.io.14egn741mv5d/v1

ASAPCRN

AI 解读

浏览 1386

Abstract

This protocol describes the fixation of cultured cells in paraformaldehyde solution and immunofluorescent labeling of mitochondria and DNA.

Attachments

HC-Protocol-Immunofluorescence.docx

Steps

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

查看全部

扫码咨询