This collection of protocols outline a working methodology to DNA barcode fungal specimens. This process will work for dried tissue, fresh tissue, or with DNA template that has already gone through an alternative extraction protocol. It takes you from computer/software setup through DNA extraction, amplification, library preparation, sequencing, and primary data analysis.

This particular workflow is designed for use with Flongle 9.4.1 flowcells and V12 Ligation chemistry.