Scalable single-cell profiling of chromatin modifications with sciCUT&Tag

Kami Ahmad, Steven Henikoff, Samuel S. Minot, Derek H. Janssens, Jacob E. Greene, Steven J. Wu, Christine A. Codomo, Scott N. Furlan

Published: 2023-11-07 DOI: 10.1038/s41596-023-00905-9

single-cell chromatin profiling

Supplementary information

Supplementary Information

Supplementary Methods and Figs. 1 and 2.

Reporting Summary

Supplementary Table 1

All the barcoded Tn5 s5 and s7 and universal MErev adapter sequences are shown in the first tab, the barcoded i5 and i7 primer sequences in the second tab and the custom primer sequences used for illumine sequencing in the third tab.

Supplementary Table 2

Layout of the 96-well sci-pA–Tn5 plate

Supplementary Table 3

Layout of the 384-well i5 and i7 primer plate for dispensing on the ICell8 instrument

Supplementary Table 4

An example of the input file for the sciCTextract custom software that assigns sequencing reads to sample name prefixes based on the s5 and s7 adapter sequence

Supplementary Table 5

An example of the input file for the sciCTextract custom software that assigns sequencing reads to sample name suffixes based on the i5 and i7 adapter sequence

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

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