Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron
Daniele Neri, Lori Zeltser, Seoeun Lee
Published: 2022-07-07 DOI: 10.17504/protocols.io.261generdg47/v1
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Abstract
A collection of protocols to sequence and analyze single neurons from the stellate ganglia of mice after injection of Cholera Toxin B (CTB) in the interscapular brown adipose fat.
Step 1: Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB)
Step 2: Ganglia dissociation and single-cell sorting
Step 3: Single-cell sequencing and analysis
Steps
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