Honey bee functional genomics using symbiont-mediated RNAi

Patrick J. Lariviere, Sean P. Leonard, Richard D. Horak, J. Elijah Powell, Jeffrey E. Barrick

Published: 2022-12-01 DOI: 10.1038/s41596-022-00778-4

Honey bee

Functional genomics

Symbiont-mediated RNAi

Gene knockdown

Engineered symbionts

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Honey bees are indispensable pollinators and model organisms for studying social behavior, development and cognition. However, their eusociality makes it difficult to use standard forward genetic approaches to study gene function. Most functional genomics
Introduction
Materials
Procedure
Timing
Troubleshooting
Anticipated results
- Reporting summary
- References
References
ACKNOWLEDGMENTS

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Honey bee functional genomics using symbiont-mediated RNAi

Supplementary information

Reporting Summary

Supplementary Video 1

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