Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Byungil Kim, Sarah Arcos, Katherine Rothamel, Manuel Ascano

Published: 2020-12-01 DOI: 10.1038/s41596-020-00429-6

Viral crosslinking

Solid-phase purification

Ribonucleoprotein complexes

RNA virus genomes

Proteomics

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The initial interactions between incoming, pre-replicated virion RNA and host protein factors are important in infection and immunity. Yet currently there are no methods to study these crucial events. We established VIR-CLASP (VIRal Cross-Linking And Soli
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

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