Von Stosch (VS) enriched seawater medium solution

Anton Kuech

Published: 2024-04-25 DOI: 10.17504/protocols.io.14egn77emv5d/v1

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Abstract

This protocol outlines the steps required to prepare von Stosch (VS) enriched seawater medium modified according to Guiry & Cunningham (1984).

Citation

M. D. Guiry & E. M. Cunningham 1984 Photoperiodic and temperature responses in the reproduction of north-eastern Atlantic Gigartina acicularis (Rhodophyta: Gigartinales) Phycologia http://doi.org/10.2216/i0031-8884-23-3-357.1

Before start

It is important to follow the order of chemicals added as shown in the protocol.

Steps

Vitamin B12 stock solution

5mg in 500mL

Note

The vitamin B12 stock solution has to be prepared separately to the primary solution and should be stored in the fridge.

Primary solution

1.1.

In 4L dissolve:

1.10.

Pasteurise for 2h 0m 0s at 99°C

1.2.

1.86g (Na₂-EDTA 2 H₂O)

1.3.

21.26g

1.4.

0.139g

1.5.

0.98g

1.6.

0.05g

1.7.

0.1g

1.8.

20mL Vitamin B12 stock solution (5mg in 500mL )

1.9.

10mg

Storage

Store VS solution in Duran bottles in a cold and dark setting at 10°C .

Equipment

Value	Label
Duran® laboratory bottles, with caps	NAME
Glass bottle	TYPE
Duran	BRAND
Z305197-10EA	SKU
http://sigmaaldrich.com	LINK
500 ml	SPECIFICATIONS

Application

Culturing medium is created by adding 200mL VS to 10L sterile seawater